Journal: Scientific Reports

Article Title: Unravelling TPX2-centered co-expression networks as key drivers of aggressive prostate cancer

doi: 10.1038/s41598-025-27704-4

Figure Lengend Snippet: Shared genes and pathways between TPX2 WGCNA modules. ( a ) Upset plot showing shared DEGs between WGCNA modules related to TPX2 as a hub gene, including M3 (prim/norm), M2 (mHSPC/prim) and M1 (mCRPC/mHSPC). A total of 22 DEGs were shared among all three modules. ( b ) Heatmap of the unsupervised hierarchical clustering analysis on the variance-stabilized transformed (VST) expression of shared DEGs among all TPX2 modules as in (a). Each row corresponds to a single gene, whereas each column corresponds to a single sample. ( c ) Upset plot showing shared REACTOME pathways between modules as in (a). ( d ) A total of 20 pathways were shared among all three modules as in (a). The circle size is the gene ratio for each shared pathway in each comparison.

Article Snippet: TMA FFPE blocks were cut into 3 μm thick sections and manual IHC staining was performed using a Rabbit Anti-Human TPX2 antibody (11741-1-AP, Proteintech) diluted at 1:200.

Techniques: Transformation Assay, Expressing, Comparison

Journal: Scientific Reports

Article Title: Unravelling TPX2-centered co-expression networks as key drivers of aggressive prostate cancer

doi: 10.1038/s41598-025-27704-4

Figure Lengend Snippet: Validation of TPX2 protein expression. ( a ) Representative microscopic images of IHC staining of normal (norm), primary PCa (prim) and lymph node metastatic (met) PCa samples. Brown nuclear staining indicates TPX2 expression. Sections were counterstained with hematoxilin (blue color). ( b ) Protein expression levels indicated by H-scores, calculated from tissue microarrays containing 154 norm, 194 prim and 54 met tissue core biopsies. ( c ) Kaplan Meyer survival curves for high (red) and low (blue) expression levels of TPX2 . The expression cutoff (6.38) was determined by recursive partitioning. Hazard ratio (HR) including 95% confidence interval (CI) were determined by Cox proportional hazards analysis.

Article Snippet: TMA FFPE blocks were cut into 3 μm thick sections and manual IHC staining was performed using a Rabbit Anti-Human TPX2 antibody (11741-1-AP, Proteintech) diluted at 1:200.

Techniques: Biomarker Discovery, Expressing, Immunohistochemistry, Staining

Journal: Scientific Reports

Article Title: Unravelling TPX2-centered co-expression networks as key drivers of aggressive prostate cancer

doi: 10.1038/s41598-025-27704-4

Figure Lengend Snippet: Univariate Cox proportional hazards analysis of TPX2 -associated genes. Forest plot shows univariate Cox regression analysis of six TPX2 -associated genes ( TPX2 , MYBL2 , EXO1 , RRM2 , CENPA , and NEIL3 ), using biochemical recurrence (BCR) as the outcome. Hazard ratios (HR) with 95% confidence intervals (CI) are displayed for high vs. low expression groups, as defined by recursive partitioning (RP) cutpoints. After FDR correction, all six genes showed significant higher risk of BCR.

Article Snippet: TMA FFPE blocks were cut into 3 μm thick sections and manual IHC staining was performed using a Rabbit Anti-Human TPX2 antibody (11741-1-AP, Proteintech) diluted at 1:200.

Techniques: Expressing

Journal: Discover Oncology

Article Title: Integrated transcriptomic and immunoinformatics analysis identifies tpx2 , bub1b , and ube2c as diagnostic biomarkers and therapeutic targets in endometrial carcinoma

doi: 10.1007/s12672-025-03800-9

Figure Lengend Snippet: Comparative mRNA expression analysis of hub genes in endometrial carcinoma (EC) tissues. A Relative expression levels of ten key genes ( aurka , bub1b , ccnb1 , cdc20 , cdk1 , chek1 , rad51 , tpx2 , tyms , and ube2c ) in EC versus normal tissues based on the GSE63678 ( n = 12; 7 EC and 5 normal) and GSE17025 ( n = 103) datasets. B Unpaired analysis of mRNA expression using the GEPIA platform, showing significant upregulation of all ten genes in EC tumor samples ( n = 147) compared to normal controls ( n = 91). C Paired analysis of tumor versus adjacent normal tissues ( n = 23 pairs) using the Xiantao platform, confirming consistent overexpression of these genes. These findings support the transcriptional upregulation of the selected hub genes in EC and reinforce their potential as diagnostic and therapeutic targets

Article Snippet: The expression of AURKA (Proteintech, Wuhan, China, A00246-4), BUB1B (Proteintech, Wuhan, China, 83920-6RR), TPX2 (Proteintech, Wuhan, China, 11741-1-AP), and UBE2C (Proteintech, Wuhan, China, 12134-2-AP) was assessed using immunohistochemistry (IHC).

Techniques: Expressing, Over Expression, Diagnostic Assay, Biomarker Discovery

Journal: Discover Oncology

Article Title: Integrated transcriptomic and immunoinformatics analysis identifies tpx2 , bub1b , and ube2c as diagnostic biomarkers and therapeutic targets in endometrial carcinoma

doi: 10.1007/s12672-025-03800-9

Figure Lengend Snippet: Immunohistochemical detection of AURKA, BUB1B, TPX2, and UBE2C expression in EC and normal endometrial tissues (400× magnification). Panels A–D represent AURKA, BUB1B, TPX2, and UBE2C expression in normal endometrial tissues, respectively; Panels E–H represent the corresponding expression in endometrial carcinoma tissues. Scale bars are included in all panels for reference. Negative controls (isotype/secondary antibody–only) were performed during staining optimization to confirm antibody specificity but are not shown here; representative examples are available upon request

Article Snippet: The expression of AURKA (Proteintech, Wuhan, China, A00246-4), BUB1B (Proteintech, Wuhan, China, 83920-6RR), TPX2 (Proteintech, Wuhan, China, 11741-1-AP), and UBE2C (Proteintech, Wuhan, China, 12134-2-AP) was assessed using immunohistochemistry (IHC).

Techniques: Immunohistochemical staining, Expressing, Staining

Journal: Discover Oncology

Article Title: Integrated transcriptomic and immunoinformatics analysis identifies tpx2 , bub1b , and ube2c as diagnostic biomarkers and therapeutic targets in endometrial carcinoma

doi: 10.1007/s12672-025-03800-9

Figure Lengend Snippet: Prognostic and diagnostic significance of hub genes in endometrial carcinoma (EC). ( A ) Kaplan–Meier survival curves showing overall survival differences in EC patients with high vs. low expression of ten hub genes. Genes such as aurka , bub1b , tpx2 , and ube2c showed significant associations with poor survival. ( B ) Receiver operating characteristic (ROC) curves evaluating the diagnostic accuracy of each hub gene for EC. All genes demonstrated strong diagnostic power, with AUC values above 0.88, and ube2c showing the highest AUC (0.971)

Article Snippet: The expression of AURKA (Proteintech, Wuhan, China, A00246-4), BUB1B (Proteintech, Wuhan, China, 83920-6RR), TPX2 (Proteintech, Wuhan, China, 11741-1-AP), and UBE2C (Proteintech, Wuhan, China, 12134-2-AP) was assessed using immunohistochemistry (IHC).

Techniques: Diagnostic Assay, Expressing

Journal: Discover Oncology

Article Title: Integrated transcriptomic and immunoinformatics analysis identifies tpx2 , bub1b , and ube2c as diagnostic biomarkers and therapeutic targets in endometrial carcinoma

doi: 10.1007/s12672-025-03800-9

Figure Lengend Snippet: Expression patterns of bub1b , tpx2 , and ube2c across clinical stages and histological grades in endometrial carcinoma (EC). A–C Box plots showing transcript levels of bub1b , tpx2 , and ube2c across clinical stages I–IV based on TCGA-UCEC data from the UALCAN platform. All three genes demonstrate consistently elevated expression across progressive tumor stages. D–F mRNA expression of the same genes analyzed by histological grade (G1–G3) using TCGA data. Gene expression levels increase with higher tumor grade, supporting their role in disease aggressiveness. All comparisons show statistical significance ( P < 0.01), highlighting the potential of bub1b , tpx2 , and ube2c as markers of both EC onset and progression. Box plots display the median, interquartile range (IQR), and whiskers representing 1.5× IQR; individual dots indicate outliers. Thus, the error bars represent data spread based on IQR rather than standard deviation (SD) or standard error of the mean (SEM)

Article Snippet: The expression of AURKA (Proteintech, Wuhan, China, A00246-4), BUB1B (Proteintech, Wuhan, China, 83920-6RR), TPX2 (Proteintech, Wuhan, China, 11741-1-AP), and UBE2C (Proteintech, Wuhan, China, 12134-2-AP) was assessed using immunohistochemistry (IHC).

Techniques: Expressing, Gene Expression, Standard Deviation

Journal: Discover Oncology

Article Title: Integrated transcriptomic and immunoinformatics analysis identifies tpx2 , bub1b , and ube2c as diagnostic biomarkers and therapeutic targets in endometrial carcinoma

doi: 10.1007/s12672-025-03800-9

Figure Lengend Snippet: Correlation between hub gene expression and immune cell infiltration in endometrial carcinoma (EC). Scatter plots generated using the TIMER 2.0 platform show the relationship between the expression levels of ( A ) bub1b , ( B ) tpx2 , and ( C ) ube2c with tumor purity and various immune cell populations, including B cells, CD8⁺ T cells, CD4⁺ T cells, macrophages, neutrophils, and dendritic cells. Partial correlation coefficients (partial.cor) and p-values indicate significant negative associations between gene expression and immune infiltration, particularly for neutrophils and dendritic cells, suggesting an immunosuppressive tumor microenvironment linked to overexpression of these genes

Article Snippet: The expression of AURKA (Proteintech, Wuhan, China, A00246-4), BUB1B (Proteintech, Wuhan, China, 83920-6RR), TPX2 (Proteintech, Wuhan, China, 11741-1-AP), and UBE2C (Proteintech, Wuhan, China, 12134-2-AP) was assessed using immunohistochemistry (IHC).

Techniques: Gene Expression, Generated, Expressing, Over Expression

Journal: Discover Oncology

Article Title: Integrated transcriptomic and immunoinformatics analysis identifies tpx2 , bub1b , and ube2c as diagnostic biomarkers and therapeutic targets in endometrial carcinoma

doi: 10.1007/s12672-025-03800-9

Figure Lengend Snippet: Correlation between mRNA expression of tpx2 and ube2c and drug sensitivity across GDSC compounds. Bubble plot illustrating the relationship between expression levels of tpx2 and ube2c and sensitivity to various anticancer agents based on the Genomics of Drug Sensitivity in Cancer (GDSC) database. The size of each bubble indicates the –log₁₀(FDR), while the color scale represents the correlation coefficient. Additionally, representative molecular docking images of trametinib with TPX2 and UBE2C are shown, with interaction types explicitly labeled: hydrogen bonds (green dashed lines), hydrophobic contacts (yellow dashed lines), and π–π interactions (purple lines). Distances are indicated in Å to provide clarity on binding modes. Drug–gene associations represent aggregated correlations across ~ 1,000 human cancer cell lines in the GDSC database; raw per–cell line IC₅₀/AUC values are not included here and can be accessed directly from the GDSC repository

Article Snippet: The expression of AURKA (Proteintech, Wuhan, China, A00246-4), BUB1B (Proteintech, Wuhan, China, 83920-6RR), TPX2 (Proteintech, Wuhan, China, 11741-1-AP), and UBE2C (Proteintech, Wuhan, China, 12134-2-AP) was assessed using immunohistochemistry (IHC).

Techniques: Expressing, Labeling, Binding Assay

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Proteome of oocyte spindle identifies Ccdc69 regulates spindle assembly like “band-tightening spell”

doi: 10.1007/s00018-025-05821-7

Figure Lengend Snippet: Overdosed Ccdc69 impairs aMTOCs distribution and spindle bipolarity. A . Relative length of spindles in control and Ccdc69-OE oocytes at different times after released from IBMX. At least 35 oocytes of control or overexpression group were examined at each timepoint. *** p < 0.001, **** p < 0.0001. B . Representative images of aMTOCs distribution during spindle formation in control and Ccdc69-OE oocytes. Red, γ-Tubulin; blue, DNA. Scale bar = 10 μm. C . Representative images of p-Aurka in control and Ccdc69-OE oocytes at MI stage. Red, p-Aurka; green, α-Tubulin; blue, DNA. Scale bar = 10 μm. D . Immunofluorescence analysis of p-Aurka in control and Ccdc69-OE oocytes. **** p < 0.0001, n = number of oocytes. E . Representative images of p-Aurka in control and Ccdc69 −/− oocytes at MI stage. Red, p-Aurka; green, α-Tubulin; blue, DNA. Scale bar = 10 μm. F . Immunofluorescence analysis of p-Aurka in control and Ccdc69-OE oocytes. ** p < 0.01, n = number of oocytes. G . Representative images of Tpx2 in control and Ccdc69-OE oocytes at MI stage. Red, Tpx2; green, α-Tubulin; blue, DNA. Scale bar = 10 μm. H . Representative images of Tacc3 in control and Ccdc69-OE oocytes at MI stage. Magenta, Tacc3; green, α-Tubulin; blue, DNA. Scale bar = 10 μm

Article Snippet: The following primary antibodies were used to detect proteins: mouse anti-α-Tubulin-FITC antibody (1:800; F2168, Sigma-Aldrich), rabbit anti-Myc-tag (1:150; AE070, ABclonal), rabbit anti-γ-tubulin (1:800; 15176-1-AP, Proteintech), rabbit anti-Tpx2 antibody (1:100, 11741-1-AP, Proteintech), rabbit anti-phospho-Aurora A (1:250; 3079 T, Cell Signaling Technology), rabbit anti-TACC3 (1:400; ab134154, Abcam).

Techniques: Control, Over Expression, Immunofluorescence